The study of fecundity and spawning patterns of three spot gourami (Trichopodus trichopterus Pallas, 1770) in Lempake Reservoir, East Kalimantan has never been reported. The purpose of this study was to analyze the fecundity of three spot gourami related to total length and body weight and its spawning patterns. A total of 30 samples of female fishes in gonad maturity conditions were collected from the Lempake reservoir from March to April 2021. To analyze its fecundity and spawning patterns were used gravimetric and measurement of egg diameter methods. The results of this research showed that the fecundity of three spot gourami from Lempake Reservoir ranged from 448-1.257 eggs/individual, with a total length of fish ranged from 82.13-112.46 mm and weights ranged from 9.1-22.5 g. The relation between fecundity with length and body weight of three spot gourami based on regression equation were F=0.0003 L^3.231 (r=0.89) and F=58.76W^1.033 (r=0.88). The diameter of the eggs in three spot gourami on gonad maturity was ranged from 0.41-0.98 mm. Based on the frequency distribution of egg diameter, it is suggested that the spawning patterns of three spot gourami were a synchronous group or total spawner. Thus, conclusion fecundity and eggs diameter of three spot gourami in the Lempake Reservoir are affected by the size of fishes and water quality.